Difference between revisions of "Part:BBa K1773005:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1773005 short</partinfo>
 
<partinfo>BBa_K1773005 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
no
+
Mutagenesis primers:
 +
 
 +
Fw: cgcacatttcccgtggaatccactcttcctcaaaagg
 +
 
 +
Rev: ccttttgaggaagagtggattccacgggaaatgtgcg
 +
 
 +
 
 +
Primers for Suffix and prefix insertion:
 +
 
 +
Fw: actgaattcgcggccgcttctagatgataactaaaacaacagtagtaaaagc
 +
 
 +
Rev: cggctgcagcggccgctactagtaattaattagaaatgtggaacggttg
 +
 
  
  

Latest revision as of 15:01, 10 September 2015

I-F type CRISPR-Cas Cascade complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1790
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2307


Design Notes

Mutagenesis primers:

Fw: cgcacatttcccgtggaatccactcttcctcaaaagg

Rev: ccttttgaggaagagtggattccacgggaaatgtgcg


Primers for Suffix and prefix insertion:

Fw: actgaattcgcggccgcttctagatgataactaaaacaacagtagtaaaagc

Rev: cggctgcagcggccgctactagtaattaattagaaatgtggaacggttg



Source

Aggregatibacter actinomycetemcomitans D-7S

References