Difference between revisions of "Part:BBa K1638009"

Revision as of 15:00, 10 September 2015

T18 domain of cyaA from Bordetella pertussis with GFP reporter

T18 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This is part is primarily used to verify correct folding of the protein fused to the T18-domain through the flexible aa linker. Correct protein folding of the fusion protein is important for the purpose of the Bacterial Two-Hybrid System.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 711
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 252
Illegal NgoMIV site found at 662
Illegal AgeI site found at 468
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1384

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 <partinfo>BBa_K1638009 short</partinfo>
-T18 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with GFP through a flexible 10 aa linker. This indirectly allows detection of correctly transcription, translation and folding of both T18 domain and GFP (by the presence of green fluorescence).
+T18 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This is part is primarily used to verify correct folding of the protein fused to the T18-domain through the flexible aa linker. Correct protein folding of the fusion protein is important for the purpose of the Bacterial Two-Hybrid System.
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