Difference between revisions of "Part:BBa K1638010"
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<partinfo>BBa_K1638010 short</partinfo> | <partinfo>BBa_K1638010 short</partinfo> | ||
| − | .. | + | T25 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This is part is primarily used to verify correct folding of the protein fused to the T25-domain through the flexible aa linker. Correct protein folding of the fusion protein is important for the purpose of the Bacterial Two-Hybrid System. |
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Revision as of 14:59, 10 September 2015
T25 domain of cyaA from Bordetella pertussis with GFP reporter
T25 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This is part is primarily used to verify correct folding of the protein fused to the T25-domain through the flexible aa linker. Correct protein folding of the fusion protein is important for the purpose of the Bacterial Two-Hybrid System.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 843
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1516