Difference between revisions of "Part:BBa K1604020"

Revision as of 14:56, 10 September 2015

araC-pBAD + beta-carotene

This device produces beta&; carotene under the control of an arabinose promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
Illegal BamHI site found at 3185
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2721
Illegal NgoMIV site found at 2851
Illegal AgeI site found at 979
Illegal AgeI site found at 1936
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1604020 short</partinfo>
-This Composite Biobrick is created by standard assembly of Part BBa_K731201 and Part BBa_K274200, which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1C3 (high copy, Chloramphenicol resistance).
+This device produces beta&; carotene under the control of an arabinose promoter.
-BBa_K274200 contains the enzymes that convert colourless farnesyl pyrophosphate to orange beta-carotene. The carotene operon contains CrtE, CrtB, CrtI and CrtY each one with individual ribosome binding site. This Composite Biobrick has been put under inducible araC-pBAD promoter that is positively regulated by arabinose.