Difference between revisions of "Part:BBa K1686046:Design"
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− | The gene sequence | + | The gene sequence comes from of GenBank : AF057142.2 |
+ | « » , Stan J.Stasinopoulos & all, 1999 | ||
+ | http://www.ncbi.nlm.nih.gov/nuccore/333794012?from=102454&to=104418&sat=37&sat_key=73052427 | ||
+ | GenBank: AECL01000028.1 | ||
+ | |||
===References=== | ===References=== | ||
− | + | Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene" Glycobiology 9:31–41 | |
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Revision as of 14:40, 10 September 2015
Curdlan synthase gene with codon optimisation for E. coli
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal AgeI site found at 1192 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene of Curdlan synthase comes from Agrobacterium and we would like to use it in E.coli, so we fixed the bias codon by using the software of Integrated DNA Technologies, Inc for codon optimization.
Source
The gene sequence comes from of GenBank : AF057142.2 « » , Stan J.Stasinopoulos & all, 1999 http://www.ncbi.nlm.nih.gov/nuccore/333794012?from=102454&to=104418&sat=37&sat_key=73052427 GenBank: AECL01000028.1
References
Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene" Glycobiology 9:31–41