Difference between revisions of "Part:BBa K1686046:Design"
Jdesca911e (Talk | contribs) (Created page with "===Design Notes=== The gene of Curdlan synthase comes from Agrobacterium and we would like to use it in E.coli, so we fixed the bias codon by using the software of ''Integrated ...") |
Jdesca911e (Talk | contribs) |
||
| Line 1: | Line 1: | ||
| + | __NOTOC__ | ||
| + | <partinfo>BBa_K1686046 short</partinfo> | ||
| + | |||
| + | <partinfo>BBa_K1686046 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | |||
===Design Notes=== | ===Design Notes=== | ||
Revision as of 14:25, 10 September 2015
Curdlan synthase gene with codon optimisation for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 678
Illegal XhoI site found at 400 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal AgeI site found at 1192 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene of Curdlan synthase comes from Agrobacterium and we would like to use it in E.coli, so we fixed the bias codon by using the software of Integrated DNA Technologies, Inc for codon optimization.
Source
The gene sequence was isolated from E. coli BL21(DE3).
References
Zeng, J., X. Lin, et al. (2011). "Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli." Appl Microbiol Biotechnol 89(6): 1841-1849.