Difference between revisions of "Part:BBa K1618028:Experience"

 
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===Applications of BBa_K1618028===
 
===Applications of BBa_K1618028===
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== NRP-UEA 2015 ==
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At NRP-UEA, we used the chloroplast transit peptide in 8 of our composite constructs in order to make sure that our parts reached the chloroplast, where starch is produced. For 4 of these constructs, we included a Yellow Fluorescent Protein tag, in order to visualise if our parts did in fact reach the chloroplast. Using a confocal microscope, we were able to get the following images, in which the chloroplast is red and our protein with the fluorescent tag is yellow.
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[[File:Confocal_Image_029-032.png]]
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''Figure 1: Constructs BBa_K1618029-032 contain a yellow fluorescent protein, as well as a chloroplast transit peptide. These are confocal microscopy images of the constructs infiltrated into ''Nicotiana benthamiana'', in which the red fluorescence is the chloroplast, and the yellow fluorescence is construct (a) BBa_K1618029, (b) BBa_K1618031, (c) BBa_K1618032, and (d) BBa_K1618030''
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The results above suggest that the chloroplast transit peptide is functional in regards to the localisation of parts to the chloroplast.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 10:06, 10 September 2015


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1618028

NRP-UEA 2015

At NRP-UEA, we used the chloroplast transit peptide in 8 of our composite constructs in order to make sure that our parts reached the chloroplast, where starch is produced. For 4 of these constructs, we included a Yellow Fluorescent Protein tag, in order to visualise if our parts did in fact reach the chloroplast. Using a confocal microscope, we were able to get the following images, in which the chloroplast is red and our protein with the fluorescent tag is yellow.

Confocal Image 029-032.png

Figure 1: Constructs BBa_K1618029-032 contain a yellow fluorescent protein, as well as a chloroplast transit peptide. These are confocal microscopy images of the constructs infiltrated into Nicotiana benthamiana, in which the red fluorescence is the chloroplast, and the yellow fluorescence is construct (a) BBa_K1618029, (b) BBa_K1618031, (c) BBa_K1618032, and (d) BBa_K1618030

The results above suggest that the chloroplast transit peptide is functional in regards to the localisation of parts to the chloroplast.

User Reviews

UNIQ566bfd140374b466-partinfo-00000000-QINU UNIQ566bfd140374b466-partinfo-00000001-QINU