Difference between revisions of "Part:BBa K1618028"
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This is synthetic a 5'UTR, omega (Tobacco Mosaic Virus. The chloroplast transit peptide provides subcellular localisation to the chloroplast. | This is synthetic a 5'UTR, omega (Tobacco Mosaic Virus. The chloroplast transit peptide provides subcellular localisation to the chloroplast. | ||
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| + | == NRP-UEA == | ||
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| + | At NRP-UEA, we used the chloroplast transit peptide in 8 of our composite constructs in order to make sure that our parts reached the chloroplast, where starch is produced. For 4 of these constructs, we included a Yellow Fluorescent Protein tag, in order to visualise if our parts did in fact reach the chloroplast. Using a confocal microscope, we were able to get the following images, in which the chloroplast is red and our protein with the fluorescent tag is yellow. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1618028 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1618028 SequenceAndFeatures</partinfo> | ||
Revision as of 09:53, 10 September 2015
Chloroplast Transit Peptide
This is synthetic a 5'UTR, omega (Tobacco Mosaic Virus. The chloroplast transit peptide provides subcellular localisation to the chloroplast.
NRP-UEA
At NRP-UEA, we used the chloroplast transit peptide in 8 of our composite constructs in order to make sure that our parts reached the chloroplast, where starch is produced. For 4 of these constructs, we included a Yellow Fluorescent Protein tag, in order to visualise if our parts did in fact reach the chloroplast. Using a confocal microscope, we were able to get the following images, in which the chloroplast is red and our protein with the fluorescent tag is yellow.