Difference between revisions of "Part:BBa K1641011"

Revision as of 06:32, 10 September 2015

Vox, recognition site of recombinase Vika

This is the recognition site of Cre-like recombinase Vika, that is "AATAGGTCTGAGAACGCCCATTCTCAGACGTATT". This recognition site is not homologous with LoxP and hence will not cross-react with Cre or other common recombinases.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without cutting activity and start code. with This deletion do not interfere the usage of the brick.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1641011 short</partinfo>
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 This is the recognition site of Cre-like recombinase Vika, that is "AATAGGTCTGAGAACGCCCATTCTCAGACGTATT". This recognition site is not homologous with LoxP and hence will not cross-react with Cre or other common recombinases.
-Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. This deletion do not interfere the usage of the brick.
+Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without cutting activity and start code. with This deletion do not interfere the usage of the brick.
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