Difference between revisions of "Part:BBa K1641005"

Revision as of 18:59, 9 September 2015

Fusion protein of Flp-EGFP-ssra, with RBS at beginning

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Flp::EGFP::ssra-tag. This part is for expression of Flp::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.

Flp is a commonly used recombinase originated from yeast, with invertase activity that turns the DNA sequence between two anti-parallel FRT sites up-side-down. A EGFP fusion at the C-term of Flp will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two part to further avoid side-effect like misfolding. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 522
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1641005 short</partinfo>
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 This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Flp::EGFP::ssra-tag. This part is for expression of Flp::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.
-Flp is a commonly used recombinase originated from yeast, with invertase activity that turns the DNA sequence between two anti-parallel FRT sites up-side-down. A EGFP fusion at the C-term of Flp will not significantly interfere the enzymatic activity. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need.
+Flp is a commonly used recombinase originated from yeast, with invertase activity that turns the DNA sequence between two anti-parallel FRT sites up-side-down. A EGFP fusion at the C-term of Flp will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two part to further avoid side-effect like misfolding. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need.