Difference between revisions of "Part:BBa K1604030"

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<i>pncB</i> encodes for the enzyme NAPRTase (nicotinic acid phosphoribosyl-transferase).  
 
<i>pncB</i> encodes for the enzyme NAPRTase (nicotinic acid phosphoribosyl-transferase).  
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It catalyzes the formation of nicotinate mono-nucleotide, a direct precursor of NAD, from NA.
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It catalyzes the formation of nicotinate mono-nucleotide, a direct precursor of NAD, from NA.<html><a href="#fn:1" id="fnref:1" title="see footnote" class="footnote">[1]</a></html> <html><a href="#fn:2" id="fnref:1" title="see footnote" class="footnote">[2]</a></html> <html><a href="#fn:3" id="fnref:1" title="see footnote" class="footnote">[3]</a></html>.
  
 
   
 
   
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<div style="text-align:center"><html><img src="https://static.igem.org/mediawiki/parts/e/ed/Unitn_pics_2015_NADpathway.png" style="width:75%;"></img></div></html>
 
<div style="text-align:center"><html><img src="https://static.igem.org/mediawiki/parts/e/ed/Unitn_pics_2015_NADpathway.png" style="width:75%;"></img></div></html>
 
<p style="width:600px; margin-left:150px; margin-bottom:60px;
 
<p style="width:600px; margin-left:150px; margin-bottom:60px;
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text-align:justify "><b>FIGURE 1. Biochemical pathway of NAD synthesis.</b> PncB gene encodes the transcription of the NARPTase which catalyzes the formation of nicotinate mono-nucleotide from nicotinic acid.</p>
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text-align:justify "><b>FIGURE 1. Biochemical pathway of NAD synthesis.</b> PncB gene encodes the transcription of the NARPTase which catalyzes the formation of nicotinate mono-nucleotide from nicotinic acid. This is considered the limiting step of the synthesis of NAD. </p>
  
  
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<div class="footnotes">
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<hr />
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<ol>
  
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<li id="fn:1">
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<p>Susana J. Berrıos-Rivera, K.-Y. San, and G. N. Bennett. (2002) Metabolic Engineering 4, 238–247</p>
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</li>
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<li id="fn:2">
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<p>Marcel G. Wubbolts, Peter Terpstra, Jan B. van Beilen, Jaap Kingma, H. A. Rene Meesters, and Bernard Witholt. (1990) JBC, 265 (29), 17665-72</p>
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</li>
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<li id="fn:3">
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<p>Ka-Yiu San, George N. Bennett, Susana J. Berríos-Rivera, Ravi V. Vadali, Yea-Tyng Yang, Emily Horton, Fred B. Rudolph, Berna Sariyar, and Kimathi Blackwood (2002) Metabolic Engineering, 4, 182–192</p>
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</li>
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</ol>
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</div>
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</html>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1604030 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1604030 SequenceAndFeatures</partinfo>

Revision as of 15:48, 9 September 2015

pncB

pncB encodes for the enzyme NAPRTase (nicotinic acid phosphoribosyl-transferase). It catalyzes the formation of nicotinate mono-nucleotide, a direct precursor of NAD, from NA.[1] [2] [3].


Usage and Biology

FIGURE 1. Biochemical pathway of NAD synthesis. PncB gene encodes the transcription of the NARPTase which catalyzes the formation of nicotinate mono-nucleotide from nicotinic acid. This is considered the limiting step of the synthesis of NAD.


  1. Susana J. Berrıos-Rivera, K.-Y. San, and G. N. Bennett. (2002) Metabolic Engineering 4, 238–247

  2. Marcel G. Wubbolts, Peter Terpstra, Jan B. van Beilen, Jaap Kingma, H. A. Rene Meesters, and Bernard Witholt. (1990) JBC, 265 (29), 17665-72

  3. Ka-Yiu San, George N. Bennett, Susana J. Berríos-Rivera, Ravi V. Vadali, Yea-Tyng Yang, Emily Horton, Fred B. Rudolph, Berna Sariyar, and Kimathi Blackwood (2002) Metabolic Engineering, 4, 182–192

</html> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1106
  • 1000
    COMPATIBLE WITH RFC[1000]