Difference between revisions of "Part:BBa K1618027"

Revision as of 14:45, 9 September 2015

GlgB-Rv3037c with IPTG-inducible promoter

Rv3037c is a genetic sequence which encodes for a putative acyltransferase enzyme which will hopefully acylate glycogen. This is preceded by GlgB which encodes for a glycogen branching enzyme which cleaves alpha-1,4 linkages in glycogen and re-anneals the cleaved strand onto the main glycogen strand to create an alpha-1,6 linkage to create a branch. Promoted by an IPTG-inducible promoter and encoded within the standard pSB1C3 vector. The bacteria are therefore chloramphenicol resistant.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 283
Illegal BamHI site found at 1491
Illegal BamHI site found at 3142
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2952
Illegal NgoMIV site found at 3125
Illegal NgoMIV site found at 3449
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 917

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 __NOTOC__
 <partinfo>BBa_K1618027 short</partinfo>
-Rv3037c encodes for a putative acyltransferase enzymes which can acylate glycogen/starch. This is preceded by GlgB which codes for a glycogen branching enzyme which cleaves alpha-1,4 linkages in glycogen and re-anneals the cleaved strand onto the main glycogen strand to create an alpha-1,6 linkage to create a branch. Promoted by an IPTG-inducible promoter and encoded within the standard pSB1C3 vector.
+Rv3037c is a genetic sequence which encodes for a putative acyltransferase enzyme which will hopefully acylate glycogen. This is preceded by GlgB which encodes for a glycogen branching enzyme which cleaves alpha-1,4 linkages in glycogen and re-anneals the cleaved strand onto the main glycogen strand to create an alpha-1,6 linkage to create a branch. Promoted by an IPTG-inducible promoter and encoded within the standard pSB1C3 vector. The bacteria are therefore chloramphenicol resistant.
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