Difference between revisions of "Part:BBa K1642004:Design"

Revision as of 12:31, 9 September 2015

SD sequence of cpcB

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Source

Synthesized.

References

[1]Abe, K., Miyake, K., Nakamura, M., Kojima, K., Ferri, S., Ikebukuro, K., & Sode, K. (2014). Engineering of a green‐light inducible gene expression system in Synechocystis sp. PCC6803. Microbial biotechnology, 7(2), 177-183.

@@ Line 6: / Line 6: @@
 ===Source===
-Ribosome binding sites(RBS) control protein expression level to a great extent. The PcpcG2 promoter does not have a typical Shine-Dalgarno (SD) sequence upstream of the start codon. Reported weak expression may be due to the lack of a SD-like sequence[1], so we add an SD sequence on the gene under PcpcG2, hoping that the gene will express on a relatively high level.
-Highly expressed photosynthesis-related proteins in cyanobacteria, the cpcB encoded c-phycocyanin, has in their gene’s 5′-UTR the SD-like sequences AGGAG respectively. The SD-like sequence from cpcB is completely complementary to the 3′ region of 16S rRNA of Synechocystis sp. PCC6803 and is highly conserved among various cyanobacteria.
+Synthesized.
-Reported about 15-fold higher GFPuv-derived fluorescence intensity resulted from the addition of the SD-like sequence to PcpcG2 proved that the SD-like sequence function strongly in Synechocystis sp. PCC6803.
 ===References===
 [1]Abe, K., Miyake, K., Nakamura, M., Kojima, K., Ferri, S., Ikebukuro, K., & Sode, K. (2014). Engineering of a green‐light inducible gene expression system in Synechocystis sp. PCC6803. Microbial biotechnology, 7(2), 177-183.