Difference between revisions of "Part:BBa K1642001:Design"
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===References=== | ===References=== | ||
| − | + | [1]Amezaga, J. M., Amtmann, A., Biggs, C. A., Bond, T., Gandy, C. J., Honsbein, A., ... & Templeton, M. R. (2014). Biodesalination: a case study for applications of photosynthetic bacteria in water treatment. Plant physiology, 164(4), 1661-1676. | |
[2]Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569. | [2]Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569. | ||
Revision as of 12:21, 9 September 2015
Codon-optimized Halorhodopsin with His-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 133
Illegal NgoMIV site found at 292
Illegal NgoMIV site found at 613
Illegal AgeI site found at 484 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
restriction
Source
Synthesized
References
[1]Amezaga, J. M., Amtmann, A., Biggs, C. A., Bond, T., Gandy, C. J., Honsbein, A., ... & Templeton, M. R. (2014). Biodesalination: a case study for applications of photosynthetic bacteria in water treatment. Plant physiology, 164(4), 1661-1676.
[2]Seki, A., Miyauchi, S., Hayashi, S., Kikukawa, T., Kubo, M., Demura, M., ... & Kamo, N. (2007). Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl− pump activity. Biophysical journal, 92(7), 2559-2569.