Difference between revisions of "Part:BBa K1642004:Design"

 
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<partinfo>BBa_K1642004 short</partinfo>
 
<partinfo>BBa_K1642004 short</partinfo>
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===Design Notes===
 
===Design Notes===
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RBS
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Ribosome binding sites(RBS) control protein expression level to a great extent. The PcpcG2 promoter does not have a typical Shine-Dalgarno (SD) sequence upstream of the start codon. Reported weak expression may be due to the lack of a SD-like sequence[], so we add an SD sequence on the gene under PcpcG2, hoping that the gene will express on a relatively high level.
  
 +
Reported about 15-fold higher GFPuv-derived fluorescence intensity resulted from the addition of the SD-like sequence to PcpcG2 proved that the SD-like sequence function strongly in Synechocystis sp. PCC6803.
  
  
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===References===
 
===References===
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Engineering of a green-light inducible gene expression system in Synechocystis sp. PCC6803

Revision as of 08:08, 9 September 2015

SD sequence of cpcB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ribosome binding sites(RBS) control protein expression level to a great extent. The PcpcG2 promoter does not have a typical Shine-Dalgarno (SD) sequence upstream of the start codon. Reported weak expression may be due to the lack of a SD-like sequence[], so we add an SD sequence on the gene under PcpcG2, hoping that the gene will express on a relatively high level.

Reported about 15-fold higher GFPuv-derived fluorescence intensity resulted from the addition of the SD-like sequence to PcpcG2 proved that the SD-like sequence function strongly in Synechocystis sp. PCC6803.


Source

RBS

References

Engineering of a green-light inducible gene expression system in Synechocystis sp. PCC6803