Difference between revisions of "Part:BBa K1582021"

 
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<partinfo>BBa_K1582021 short</partinfo>
 
<partinfo>BBa_K1582021 short</partinfo>
  
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This part bases on BBa_K1582020. For enhancing success rate and expression of fusion protein, we mutate of eight Cys to Ser because of their resemble structure. After doing some similar experiment, we can ensure the function of mutation that whether it can better express and fold in E. coli and would it have the same function for hydrolyzing PET
 
  
 
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Revision as of 05:30, 9 September 2015

FsC+sJanus-m Fusion Protein


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 907
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 301
    Illegal SapI site found at 916