Difference between revisions of "Part:BBa K1582005"

 
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<partinfo>BBa_K1582005 short</partinfo>
 
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HFBI is a modle of class I of hydrophobins, which are naturally produced by filamentous fungi. We use serine, which is similar with cysteine in structure, to replace all of its conserved eight-cysteine residues and get the mutant of HFBI. Hydrophobins are amphipathic, which means they have the hydrophobic part and hydropholic part at the same time. HFBI is a kind of hydrophobins and it has magic properties. We combine GFP with HFBI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.
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Revision as of 05:02, 9 September 2015

GFP+sJanus-m Fusion Protein


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 976
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SapI site found at 985