Difference between revisions of "Part:BBa S03639:Design"
(→Design Notes) |
|||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed pBad promoter followed by a reversed RBS-Tet coding region. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Latest revision as of 02:51, 31 January 2007
(-1,-2) HixC-pBadrev-HixC : TetB-RBSrev-HixC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 35
Illegal NheI site found at 1249 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 95
Illegal BamHI site found at 1103 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 549
Illegal NgoMIV site found at 709
Illegal NgoMIV site found at 1077 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed pBad promoter followed by a reversed RBS-Tet coding region.