Difference between revisions of "Part:BBa K1723001"

Revision as of 12:13, 8 September 2015

PAM rich URS j23117 promoter

PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004). All this system is operating in the special cell strain JEN202. In our experiments this promoter is 27 base pairs upstream the ATG start codon, an average of 30bp is recommended.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 56
Illegal NheI site found at 79
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 43
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1723001 short</partinfo>
-PAM rich URS J23117 is a promoter made from the existing J23117 promoter. This promoter has PAM rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).
+PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).
 All this system is operating in the special cell strain JEN202. In our experiments this promoter is 27 base pairs upstream the ATG start codon, an average of 30bp is recommended.