Difference between revisions of "Part:BBa K1732004"

Revision as of 03:36, 8 September 2015

pSB1C3-J23100-B0034-CDcel-Gaussia-His-B0015

J23100 constitutive promoter (BBa_J23100), a RBS (BBa_B0034), CDcel domain (BBa_K1732002), Gaussia luciferase (BBa_K1732003), and B0015 terminator (BBa_B0015). ______________________________________________________________________________________________________________________________

This DNA sequence, expressed from a constitutive, strong promoter allows for the Gaussia luciferase protein to be synthesized and was targeted extrecellularly into the media. The Gaussia luciferase proteins were reacted with the optimal concentration of coelenterazine to express luminescence, which was used to test the Carnegie Mellon DIY luminometer. The sequence was codon optimized for E.coli and contains a 6X His-tag for easier purification of the protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1517
Illegal BamHI site found at 975
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 715

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	This DNA sequence, expressed from a constitutive, strong promoter allows for the Gaussia luciferase protein to be synthesized and was targeted extrecellularly into the media. The Gaussia luciferase proteins were reacted with the optimal concentration of coelenterazine to express luminescence, which was used to test the Carnegie Mellon DIY luminometer. The sequence was codon optimized for E.coli and contains a 6X His-tag for easier purification of the protein.		This DNA sequence, expressed from a constitutive, strong promoter allows for the Gaussia luciferase protein to be synthesized and was targeted extrecellularly into the media. The Gaussia luciferase proteins were reacted with the optimal concentration of coelenterazine to express luminescence, which was used to test the Carnegie Mellon DIY luminometer. The sequence was codon optimized for E.coli and contains a 6X His-tag for easier purification of the protein.
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		+	[[File:CDcel Gaussia coleneterazine.jpg]]