Difference between revisions of "Part:BBa K1824005"

Latest revision as of 11:24, 6 September 2015

pBAD + RNA Thermometer U6

This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer U6 BBa_K1824563 for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

Figure 1: Possible secondary structure of RNAT U6.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 153
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
Illegal SpeI site found at 153
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
Illegal BamHI site found at 139
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 153
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 153
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1824005 short</partinfo>
-This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer U6 BBa_K1824563 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
+This is a composite regulatory part that consisting of pBAD <partinfo>BBa_I13453</partinfo> promoter and RNA Thermometer U6 <partinfo>BBa_K1824563</partinfo> for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
+[[Image:U6.jpg|450px]]
+<br>'''Figure 1:''' Possible secondary structure of RNAT U6.
 <!-- Add more about the biology of this part here