Difference between revisions of "Part:BBa K1592001"

(Created page with "This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3. LIP prepro is signal peptide used to secrete the interest protein ou...")
 
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This is the cell display system of Yarrowia lipolytica, composed of LIP prepro, interest protein, and YLcwp3.
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Mcfp-3 is foot protein secreted from Mytilus californianus. The protein is of significance to the formation of byssus to help mussels permanently or temporarily tether to the surface of solid surface of reef or ship-body. This coherent substance shows the excellent adhesion performance with no substitute under water for sustaining the repeating wash of waves and having no toxicity. The properties are owing to the amino acid content which are successively DOPA (28mol％),glycine,lysine and asparagine[*]. The presence of DOPA in the foot protein suggests a role in protein-protein and protein-substrate(glass eg.) interactions related to the formation of adhesive plaques. The electron rich nature of DOPA and its susceptibility to oxidation can lead to the formation of o-quinone with subsequent tanning or sclerotization to form a cross-linked and stable products. Further,it is noteworthy that the protein shows an intrinsic ability of self-assemble into stable,higher molecular weight forms that may further enhance our consolidation function.
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LIP prepro is signal peptide used to secrete the interest protein out of the cell, and the YLcwp3 is the anchor domain binding the interest protein to the cell wall of yeast.
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We use this system to display silica-tag and test its binding characteristics.  
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Reference:
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E.coli ribosomal protein L2 was found to bind tightly to silicon particles, which have surfaces that are oxidized to silica. This L2 silica-binding tag, called the ‘‘Si-tag,’’ can be used for one-step targeting of functional proteins on silica surfaces. The silica-binding domains of E. coli L2 was mapped to amino acids 1–60, 61-202 and 203–273. We respectively test the silica-binding characteristics of this three regions and their combinations. E. coli ribosomal protein L2 (1-60) is the amino acids 1–60 of E. coli ribosomal protein L2, we call it si-tag1.
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ZHAOH, ROBERTSONNB, JEWHURSTSA, et al. Probing the adhesive footprints of Mytilus Californianus byssus [J]. JBiol (16):11090-11096. Chem, 2006, 281

Revision as of 02:45, 6 September 2015

Mcfp-3 is foot protein secreted from Mytilus californianus. The protein is of significance to the formation of byssus to help mussels permanently or temporarily tether to the surface of solid surface of reef or ship-body. This coherent substance shows the excellent adhesion performance with no substitute under water for sustaining the repeating wash of waves and having no toxicity. The properties are owing to the amino acid content which are successively DOPA (28mol％),glycine,lysine and asparagine[*]. The presence of DOPA in the foot protein suggests a role in protein-protein and protein-substrate(glass eg.) interactions related to the formation of adhesive plaques. The electron rich nature of DOPA and its susceptibility to oxidation can lead to the formation of o-quinone with subsequent tanning or sclerotization to form a cross-linked and stable products. Further,it is noteworthy that the protein shows an intrinsic ability of self-assemble into stable,higher molecular weight forms that may further enhance our consolidation function.

Reference: ZHAOH, ROBERTSONNB, JEWHURSTSA, et al. Probing the adhesive footprints of Mytilus Californianus byssus [J]. JBiol (16):11090-11096. Chem, 2006, 281