Difference between revisions of "Part:BBa K1636000:Design"

 
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<partinfo>BBa_K1636000 short</partinfo>
 
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===References===
 
===References===
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Li T., Dong S., and Wang E. (2010) A Lead (II)-Driven DNA Molecular Device for Turn-On Fluorescence Detection of Lead(II) Ion with High Selectivity and Sensitivity. J. Am. Chem. Soc., 132 (38), pp 13156–13157

Revision as of 23:44, 5 September 2015

Lead-II-ions-specific aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For standardisation purposes and its usage in iGEM, this sequence was cloned as a duplex on pSB1C3 thereby adding the prefix and suffix sequences to it. Given the fact that the sequence is need as a single-strand molecule, a primer was designed in order to carry out a PCR with a single primer for the production of this molecule.


Source

This sequence does not come from a genomic sequence. First reported by Li et al 2010, it is comprised by 16 nucleotides with a high percentage of G which are the ones capable of stabilising and forming the quadruplex with the lead ion.


References

Li T., Dong S., and Wang E. (2010) A Lead (II)-Driven DNA Molecular Device for Turn-On Fluorescence Detection of Lead(II) Ion with High Selectivity and Sensitivity. J. Am. Chem. Soc., 132 (38), pp 13156–13157