Difference between revisions of "Part:BBa K1699004:Design"

(→‎References)
(→‎References)
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===References===
 
===References===
−
3. In vivo genome editing using Staphylococcus aureus Cas9.  
+
1. In vivo genome editing using Staphylococcus aureus Cas9.  
 
<br />http://10.1038/nature14299
 
<br />http://10.1038/nature14299

Revision as of 19:47, 5 September 2015


gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design


Source

Source

References

1. In vivo genome editing using Staphylococcus aureus Cas9.
http://10.1038/nature14299