Difference between revisions of "Part:BBa K1699004"
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===Characterization===
 
===Characterization===
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characterization
 
  
 
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Revision as of 19:26, 5 September 2015

gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter

gRNA for SaCas9 targeting ubiquitin B under U6 promoter. This gRNA sequence complements 3 different loci in the second exon of human UBB gene, and is under U6 (RNA pol III) promoter.


Usage and Biology

usage and biology


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]