Difference between revisions of "Part:BBa K1824004"
 
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This is a composite regulatory part that consisting of pBAD <partinfo>BBa_I13453</partinfo> promoter and RNA Thermometer FourU <partinfo>BBa_K1824560</partinfo> for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
 
This is a composite regulatory part that consisting of pBAD <partinfo>BBa_I13453</partinfo> promoter and RNA Thermometer FourU <partinfo>BBa_K1824560</partinfo> for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
  
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[[Image:XJTLU-FourU.jpg|360px]]
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[[Image:XJTLU-FourU.jpg|450px]]
 
<br>'''Figure 1:''' Possible secondary structure of RNAT FourU.
 
<br>'''Figure 1:''' Possible secondary structure of RNAT FourU.
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===Characterization===
  
 
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Latest revision as of 17:47, 5 September 2015

pBAD + RNA Thermometer FourU

This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer FourU BBa_K1824560 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

XJTLU-FourU.jpg
Figure 1: Possible secondary structure of RNAT FourU.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]