Difference between revisions of "Part:BBa K1824000"
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<partinfo>BBa_K1824000 short</partinfo> | <partinfo>BBa_K1824000 short</partinfo> | ||
| − | This is a composite regulatory part that consisting of constitutive promoter <partinfo>J23119</partinfo> and RNA Thermometer | + | This is a composite regulatory part that consisting of constitutive promoter <partinfo>J23119</partinfo> and RNA Thermometer FourU <partinfo>BBa_K1824559</partinfo> for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted. |
| − | [[Image: | + | [[Image:XJTLU-FourU.jpg|450px]] |
| − | <br>'''Figure 1:''' Possible secondary structure of RNAT | + | <br>'''Figure 1:''' Possible secondary structure of RNAT FourU. |
===Characterization=== | ===Characterization=== | ||
Revision as of 15:50, 5 September 2015
Constitutive Promoter J23119 + RNA Thermometer A1
This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer FourU BBa_K1824559 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
Figure 1: Possible secondary structure of RNAT FourU.
Characterization
References
1. Nocker, A. et al. mRNA-based thermosensor controls expression of rhizobial heat shock genes. Nucleic Acids Res. 29, 4800–4807 (2001).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]