Difference between revisions of "Part:BBa K1824003"

 
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<partinfo>BBa_K1824003 short</partinfo>
 
<partinfo>BBa_K1824003 short</partinfo>
  
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This is a composite regulatory part that consisting of pBAD promoter and RNA Thermometer A1 BBa_K1824556 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
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This is a composite regulatory part that consisting of pBAD promoter and RNA Thermometer A1 <partinfo>BBa_K1824557</partinfo> for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
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[[Image:A1 RNA Thermometer.jpg|360px]]
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<br>'''Figure 1:''' Possible secondary structure of RNAT A1.
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===Characterization===
  
 
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Revision as of 15:24, 5 September 2015

pBAD + RNA Thermometer A1

This is a composite regulatory part that consisting of pBAD promoter and RNA Thermometer A1 BBa_K1824557 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.


A1 RNA Thermometer.jpg
Figure 1: Possible secondary structure of RNAT A1.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]