Difference between revisions of "Part:BBa K1595000:Design"

Line 11: Line 11:
  
 
===Source===
 
===Source===
Synthesized by Integrated DNA Technology
+
Synthesized by Integrated DNA Technologies
  
 
===References===
 
===References===
 
NCBI Reference number 132339405
 
NCBI Reference number 132339405

Revision as of 15:22, 5 September 2015

EcnB from Ralstonia pickettii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 123
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 123
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 123
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use in conjunction with strain-expressing T7 polymerase

Has internal PstI cutsite.

Source

Synthesized by Integrated DNA Technologies

References

NCBI Reference number 132339405