Difference between revisions of "Part:BBa K1613017"

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__NOTOC__
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<partinfo>BBa_K1613017 short</partinfo>
  
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This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP.
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The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1613017 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1613017 parameters</partinfo>
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Revision as of 13:42, 5 September 2015

K1613017 is a new part which can detect lead (Pb) ions in the liquid.

This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1281
    Illegal AgeI site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 686