Difference between revisions of "Part:BBa K1613017"
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| − | <partinfo>BBa_K1613017 short</ | + | <partinfo>BBa_K1613017 short</K1613017 is a new part which can detect lead (Pb) ions in the liquid. > |
This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. | This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. | ||
| − | The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive | + | The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function. |
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Revision as of 13:23, 5 September 2015
BBa_K1613017 short
This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.
Sequence and Features