Difference between revisions of "Part:BBa K1595000:Design"

(Design Notes)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1595000 short</partinfo>
 
<partinfo>BBa_K1595000 short</partinfo>
Line 12: Line 11:
  
 
===Source===
 
===Source===
 
 
Synthesized by Integrated DNA Technology
 
Synthesized by Integrated DNA Technology
  
 
===References===
 
===References===
 
NCBI Reference number 132339405
 
NCBI Reference number 132339405

Revision as of 08:58, 5 September 2015

EcnB from Ralstonia pickettii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 123
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 123
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 123
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use in conjunction with strain-expressing T7 polymerase

Has internal PstI cutsite.

Source

Synthesized by Integrated DNA Technology

References

NCBI Reference number 132339405