Difference between revisions of "Part:BBa K1824000"
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<partinfo>BBa_K1824000 short</partinfo>
 
<partinfo>BBa_K1824000 short</partinfo>
  
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This was a composite regulatory part that consisting of constitutive promoter J23119 and RNA Thermometer A1 for temperature induced gene  expression at 42 degrees<partinfo>BBa_K1824556</partinfo>. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination was compatible with the assembly method that XJTLU-CHINA promoted.
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This was a composite regulatory part that consisting of constitutive promoter J23119 and RNA Thermometer A1 <partinfo>BBa_K1824556</partinfo> for temperature induced gene  expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination was compatible with the assembly method that XJTLU-CHINA promoted.
  
  

Revision as of 20:33, 4 September 2015

Constitutive Promoter J23119 + RNA Thermometer A1

This was a composite regulatory part that consisting of constitutive promoter J23119 and RNA Thermometer A1 BBa_K1824556 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination was compatible with the assembly method that XJTLU-CHINA promoted.


A1 RNA Thermometer.jpg
Figure 1: Possible secondary structure of RNAT A1.


References

1. Nocker, A. et al. mRNA-based thermosensor controls expression of rhizobial heat shock genes. Nucleic Acids Res. 29, 4800–4807 (2001).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]