Difference between revisions of "Part:BBa K1824556"
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<partinfo>BBa_K1824556 short</partinfo>
 
<partinfo>BBa_K1824556 short</partinfo>
  
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A1 RNA thermometer was derived from the ROSE family (ROSE stands for Repression Of heat Shock gene Expression), first discovered in the 5&#8242; untranslated region of rhizobial heat shock genes (Nocker, 2001). The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). For testing results of A1 RNAT, See below.
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A1 RNA thermometer have the hairpin structure that harbors the Shine-Dalgarno sequence (SD sequence) and, in this way, make it inaccessible to the 30S unit of the bacterial ribosome, resulting in translational inactivation. The melting temperature of this RNA thermometer is 42 Celsius degree. Once reaching the melting temperature, hairpin structure would vanish and as a result, exposing the SD sequence to trigger the translation process. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). For testing results of A1 RNAT, See below.
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[[Image:A1 RNA Thermometer.jpg|360px]]
 
[[Image:A1 RNA Thermometer.jpg|360px]]
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<br>'''Figure 1:''' Possible secondary structure of RNAT A1.
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[[Image:Rna_thermometer.png|500px]]
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<br>'''XJTLU-CHINA:'''''Left picture:'' '''Figure 1:'''Possible secondary structure of RNAT A1.'''TuDelft:'''''Right picture:'' '''Figure 2:'''Responsiveness of mRNA structures to environmental cues.
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===Source===
  
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A1 RNA thermometer was derived from the ROSE family (ROSE stands for Repression Of heat Shock gene Expression).
  
  

Revision as of 20:08, 4 September 2015

RNA Thermometer A1 (Specially designed for J23119)

A1 RNA thermometer have the hairpin structure that harbors the Shine-Dalgarno sequence (SD sequence) and, in this way, make it inaccessible to the 30S unit of the bacterial ribosome, resulting in translational inactivation. The melting temperature of this RNA thermometer is 42 Celsius degree. Once reaching the melting temperature, hairpin structure would vanish and as a result, exposing the SD sequence to trigger the translation process. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). For testing results of A1 RNAT, See below.


A1 RNA Thermometer.jpg 500px
XJTLU-CHINA:Left picture: Figure 1:Possible secondary structure of RNAT A1.TuDelft:Right picture: Figure 2:Responsiveness of mRNA structures to environmental cues.


Source

A1 RNA thermometer was derived from the ROSE family (ROSE stands for Repression Of heat Shock gene Expression).


References

1. Nocker, A. et al. mRNA-based thermosensor controls expression of rhizobial heat shock genes. Nucleic Acids Res. 29, 4800–4807 (2001).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]