Difference between revisions of "Part:BBa K1620007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed. | + | This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed. |
| − | + | ||
| + | [[File: Alignment between improved SmtA and BBa_K519010.jpg]] | ||
===Source=== | ===Source=== | ||
Revision as of 14:35, 4 September 2015
Synechococcus metallothionenin A (SmtA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 121
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.
Source
This part is an improvement of part BBa_K519010. It was synthesized by IDT as G-block and amplified by PCR using the following primers:
Fwd: 5' - GAA TTC GCG GCC GCT TCT AGA GAT GAC CTC AAC AAC GTT GGT - 3'
Rev: 5' - TAC TAG TAG CGG CCG CTG CAG TTA GCC GTG GCA GTT ACA GC - 3'
After this, the 3A assembly method was carried out.