Difference between revisions of "Part:BBa K1720002:Design"
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| − | + | ===Design Notes:=== | |
We cloned this basic part into a psb1c3 vector, this part contain a hypoxia-responsive element and CMV promoter.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism. | We cloned this basic part into a psb1c3 vector, this part contain a hypoxia-responsive element and CMV promoter.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism. | ||
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| + | ===References=== | ||
1.Rational design of minimal hypoxia-inducible enhancers | 1.Rational design of minimal hypoxia-inducible enhancers | ||
Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge | Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge | ||
Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA | Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA | ||
Revision as of 13:20, 4 September 2015
Design Notes:
We cloned this basic part into a psb1c3 vector, this part contain a hypoxia-responsive element and CMV promoter.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism.
References
1.Rational design of minimal hypoxia-inducible enhancers Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA