Difference between revisions of "Part:BBa K1592006:Design"

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===References===
 
===References===
 +
Hughes R M, Bolger S, Tapadia H, et al. Light-mediated control of DNA transcription in yeast[J]. Methods, 2012, 58(4): 385-391.

Latest revision as of 11:08, 4 September 2015


GalAD-CIB1 Fusion for Yeast-Two-Hybrid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
    Illegal XhoI site found at 1474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
    Illegal AgeI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

Part cloned from pRMH120 of Addgene.

References

Hughes R M, Bolger S, Tapadia H, et al. Light-mediated control of DNA transcription in yeast[J]. Methods, 2012, 58(4): 385-391.