Difference between revisions of "Part:BBa K1732001:Design"
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The sequence is codon optimized for E.coli. | The sequence is codon optimized for E.coli. | ||
| − | The plasmid was used to produce Gaussia princeps luciferase proteins that would express luminescence when reacted with an optimal concentration of 1 uM coelenterazine. The CDcel domain allowed for the proteins to be secreted into the media (extracellular) which was both the expected and observed location. | + | The plasmid was used to produce Gaussia princeps luciferase proteins that would express luminescence when reacted with an optimal concentration of 1 uM coelenterazine. The CDcel domain allowed for the proteins to be secreted into the media (extracellular) which was both the expected and observed location. |
| − | + | ||
| + | [[File:CDcel Gaussia Sequence.jpg]] | ||
===Source=== | ===Source=== | ||
Latest revision as of 08:43, 3 September 2015
J23100-CDcel-GlucCO-B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 8
Illegal NheI site found at 31 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1614
Illegal BamHI site found at 1065 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 805
Design Notes
The sequence is codon optimized for E.coli.
The plasmid was used to produce Gaussia princeps luciferase proteins that would express luminescence when reacted with an optimal concentration of 1 uM coelenterazine. The CDcel domain allowed for the proteins to be secreted into the media (extracellular) which was both the expected and observed location.
Source
Provided by the Bruchez lab at Carnegie Mellon University.