Difference between revisions of "Part:BBa K1722005"

Revision as of 05:33, 3 September 2015

Rluc can express Renilla luciferase（Rluc）.

Rluc can express Renilla luciferase which has become popular as a reporter enzyme for gene expression assays. Renilla luciferase(RLUC) is a blue-light emitting luciferase of marine anthozoan Renilla reniformis.[1] As a reporter gene, researchers attach it to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. RLUC is chosen as a reporter because the characteristic it confer on organisms expressing it is easily identified and measured. The bioluminescence of the sea pancy, is under the control of a nerve network[2-4] and is stimulated by changes of intracellular Ca2+ concerntration.[5-7] RLUC catalyzes the oxidation of coelenteramide, CO2 and light(480nm）,as in the following scheme.

2015 SZU-iGEM construct Rlu with one codon being amber mutated and SV40, the promoter in the same plasmid to introduce Rluc into our system. This plasmid with Rluc, together with two other plasmids, are inserted into the cell. Only when the two promoters in plasmids without Rluc are activated simultanuously to express the downstream DNA sequence can Rluc being translated completely. RLUC that is produced inside the cells catalyzes a reaction with luciferin to produce light. By measuring the light intensity using Luminometer, we can tell the working efficiency of our system under different conditions.

Sequence and Features