Difference between revisions of "Part:BBa K1110003:Design"

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===References===
 
===References===
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Sequence from Diochot, Sylvie, et al. "Black mamba venom peptides target acid-sensing ion channels to abolish pain." Nature 490.7421 (2012): 552-555. Synthesized by IDT
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Mambalgin peptide coding sequence was retrieved from supplementary materials in Diochot, Sylvie, et al. "Black mamba venom peptides target acid-sensing ion channels to abolish pain." Nature 490.7421 (2012): 552-555.

Revision as of 15:05, 1 September 2015

Mambalgin-1 cDNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the design, we used assembly standard 25 and removed a start codon from the sequence. Using the pGAPZα expression system, the GSU team has been working to express the protein in Pichia Pastoris. The pGAPZα vector includes an alpha secretion, MYC epitome, and 6xHis tag region. This sequence is optimized for this expression system.

Source

Dendroaspis polylepis Synthesized by IDT

References

Mambalgin peptide coding sequence was retrieved from supplementary materials in Diochot, Sylvie, et al. "Black mamba venom peptides target acid-sensing ion channels to abolish pain." Nature 490.7421 (2012): 552-555.