Difference between revisions of "Part:BBa K1722004"
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The aminoacyl-tRNA came from barchaebacteria,and it is generally applied to unnatural amino acid orthogonal system. | The aminoacyl-tRNA came from barchaebacteria,and it is generally applied to unnatural amino acid orthogonal system. | ||
In our project, the aminoacyl-tRNA was selected by directed evolution. | In our project, the aminoacyl-tRNA was selected by directed evolution. | ||
| + | ===The structure of tRNA=== | ||
| + | Aminoacyl-tRNA only carries a specific amino acid---- Acetyllysine(ACK),and compared with the classical tRNA has an unusual secondary structure.The structure shows in Fig.1.adds in the protein by Semantic coding of termination codon(UAG) | ||
[[File:TRNA.png|300px|center]] | [[File:TRNA.png|300px|center]] | ||
| − | + | ===Acetyllysine(ACK) & Lysine=== | |
| − | + | Acetyllysine(ACK) is one of unnatural amino acid and only corresponds to a special aminoacyl-tRNA(tRNA). It can be added to the protein by Semantic coding of termination codon(UAG). | |
| − | ===Acetyllysine(ACK)=== | + | [[File:Ack.png|300px|center]] |
| − | Acetyllysine(ACK) is one of unnatural amino acid and only corresponds to a special aminoacyl-tRNA( | + | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:43, 1 September 2015
This is a kind of aminoacyl-tRNA(aa-tRNA).
This is a kind of aminoacyl-tRNA, which has a diffeent structure compared with classic tRNA.The tRNA can only carry a specific amino acid, and is non natural amino acids.It has special anticodons(CUA),which can identify the termination codon(UAG), and then adds the unnatural amino acid carried own into the polypeptide chain to.So that the position of the termination codon corresponds to the insertion of an unnatural amino acid, which allows the protein to continue to be translated. The aminoacyl-tRNA came from barchaebacteria,and it is generally applied to unnatural amino acid orthogonal system. In our project, the aminoacyl-tRNA was selected by directed evolution.
The structure of tRNA
Aminoacyl-tRNA only carries a specific amino acid---- Acetyllysine(ACK),and compared with the classical tRNA has an unusual secondary structure.The structure shows in Fig.1.adds in the protein by Semantic coding of termination codon(UAG)
Acetyllysine(ACK) & Lysine
Acetyllysine(ACK) is one of unnatural amino acid and only corresponds to a special aminoacyl-tRNA(tRNA). It can be added to the protein by Semantic coding of termination codon(UAG).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]