Difference between revisions of "Part:BBa K1666000"
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Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of ''Salmonella Typhimurium'', AI-2 response involves ATP binding cassette transporter encoded by genes named ''Lsr'' (LuxS regulated). And LsrA is the ATP-binding protein which provides energy for AI-2 transport. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of'' Lactobacillus'' or ''Lactococcus'' for the final purpose of constructing an integrated AI-2 response pathway of ''Salmonella'' in the engineered bacteria.
 
Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of ''Salmonella Typhimurium'', AI-2 response involves ATP binding cassette transporter encoded by genes named ''Lsr'' (LuxS regulated). And LsrA is the ATP-binding protein which provides energy for AI-2 transport. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of'' Lactobacillus'' or ''Lactococcus'' for the final purpose of constructing an integrated AI-2 response pathway of ''Salmonella'' in the engineered bacteria.
  
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===Usage and Biology===
 
===Usage and Biology===
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[[File:NEFU_China_2015_AI-2_response_in_Salmonalla.png|550px|thumb|center|'''Fig1. Schematic overview of the AI-2 response pathway in ''Salmonella Typhimurium''''']]
  
 
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Revision as of 03:56, 31 August 2015

LsrA of LuxS/AI-2 signaling pathway in Salmonalla

Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And LsrA is the ATP-binding protein which provides energy for AI-2 transport. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of Lactobacillus or Lactococcus for the final purpose of constructing an integrated AI-2 response pathway of Salmonella in the engineered bacteria.

Usage and Biology

Fig1. Schematic overview of the AI-2 response pathway in Salmonella Typhimurium

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 939
  • 1000
    COMPATIBLE WITH RFC[1000]