Difference between revisions of "Part:BBa K1666000:Design"

 
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===Source===
 
===Source===
  
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It comes from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2(Gene ID: 1255601)
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We extracted the genomic DNA of ''Salmonella enterica subsp. enterica serovar Typhimurium str. LT2'', and used PCR to isolate the gene ''LsrA''(Gene ID: 1255601).
  
 
===References===
 
===References===

Revision as of 02:15, 31 August 2015


LsrA of LuxS/AI-2 signaling pathway in Salmonalla


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 939
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lsrA gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR.We added restriction enzyme sites when designing primers,and the assembly is done through the use of restriction sites (cutting and ligating).


Source

We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrA(Gene ID: 1255601).

References