Difference between revisions of "Part:BBa K1621007:Design"
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Meyer T, Schirrmann T, Frenzel A, Miethe S, Stratmann-Selke J, Gerlach GF, Strutzberg-Minder K, Dübel S, Hust M: Identification of immunogenic proteins and generation of antibodies against ''Salmonella'' Typhimurium using phage display (2012). ''BMC Biotechnology'' 12:29. | Meyer T, Schirrmann T, Frenzel A, Miethe S, Stratmann-Selke J, Gerlach GF, Strutzberg-Minder K, Dübel S, Hust M: Identification of immunogenic proteins and generation of antibodies against ''Salmonella'' Typhimurium using phage display (2012). ''BMC Biotechnology'' 12:29. | ||
Revision as of 13:09, 29 August 2015
anti-dihydroxyacid dehydratase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
At position 15 a base change from guanine to thymine was inserted by site-directed mutagenesis to remove the recognition site for PstI without causing a change in the amino acid sequence.
Source
The part's sequence as well as an expression plasmid carrying a His-tagged variant was obtained from the group of Prof. Dr. Hust (TU Braunschweig).
References
Meyer T, Schirrmann T, Frenzel A, Miethe S, Stratmann-Selke J, Gerlach GF, Strutzberg-Minder K, Dübel S, Hust M: Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display (2012). BMC Biotechnology 12:29.