Difference between revisions of "Part:BBa K1755002"
Line 3: Line 3:
  
 
ribB with double terminators
 
ribB with double terminators
−
   B1755002 consisting of K1755001 and B0014 which were also characterized as ribB coding sequence and double terminators.
+
   B1755002 consisting of K1755001 and B0014 which were also characterized  
 +
  as ribB coding sequence and double terminators.
 
   ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase, which able cell produce more riboflavin.
 
   ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase, which able cell produce more riboflavin.
−
   Double terminator consisting of BBa_B0012 and BBa_B0011 that make it become the transcriptional terminator and bidirectional transcriptional terminator for the E.coli RNA polymerase.
+
   Double terminator consisting of BBa_B0012 and BBa_B0011 that make it become
 +
  the transcriptional terminator and bidirectional transcriptional terminator for
 +
  the E.coli RNA polymerase.
  
 
This part is based on part K1755001. [https://parts.igem.org/Part:BBa_K1755001  click here for more info]
 
This part is based on part K1755001. [https://parts.igem.org/Part:BBa_K1755001  click here for more info]

Revision as of 09:45, 28 August 2015

ribB+B0015

ribB with double terminators 

 B1755002 consisting of K1755001 and B0014 which were also characterized 
 as ribB coding sequence and double terminators.
 ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase, which able cell produce more riboflavin.
 Double terminator consisting of BBa_B0012 and BBa_B0011 that make it become  
 the transcriptional terminator and bidirectional transcriptional terminator for
 the E.coli RNA polymerase.

This part is based on part K1755001. click here for more info

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]