Difference between revisions of "Part:BBa K1755003"
Ritianjiang (Talk | contribs) |
Ritianjiang (Talk | contribs) |
||
| Line 14: | Line 14: | ||
In our project, we havea applied kinds of promoters | In our project, we havea applied kinds of promoters | ||
| − | which are sensitive to the concentration of copper heavy metal ions such as Cu2+ Hg2+ and Cr6+ to regulate the expression of protein ribB. [[ | + | which are sensitive to the concentration of copper heavy metal ions such as Cu2+ Hg2+ and Cr6+ to regulate the expression of protein ribB. [[File:K17553003ZUOTU.png]] |
The existence of ribB will enhance the production of riboflavin. | The existence of ribB will enhance the production of riboflavin. | ||
Revision as of 06:29, 28 August 2015
strong RBS + K1755002
This part was a constitutive expression element for ribB.
https://parts.igem.org/Part:BBa_K1755001#references
This part consisting of B0032 and B0014 which were characterized as
ribB coding sequence and double terminators.
Usage in MFC
In our project, we havea applied kinds of promoters
which are sensitive to the concentration of copper heavy metal ions such as Cu2+ Hg2+ and Cr6+ to regulate the expression of protein ribB. 
The existence of ribB will enhance the production of riboflavin.
Moreover, riboflavin, as the electron carrier, could improve the electricity producing performance of the Microbial Fuel Cells (MFCs).
So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC.
Usage and Biology
When ribB coding sequence had RBS and terminator,we could regulate the expression of protein ribB with different promoters.