Difference between revisions of "Part:BBa K1598004:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1598004 short</partinfo>
 
<partinfo>BBa_K1598004 short</partinfo>
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- human CHAT gene (UniProt accession no: Q99259) was codon-optimized for E.coli and provided with C-terminal 6xHis tag
 
- human CHAT gene (UniProt accession no: Q99259) was codon-optimized for E.coli and provided with C-terminal 6xHis tag
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<br>
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- illegal restriction enzyme sites were removed <br>
  
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- illegal restriction enzyme sites were removed
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- part was obtained through de novo DNA synthesis<br><br>
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<img src="https://static.igem.org/mediawiki/2015/f/fb/UCL_MG_6280.jpg" style="width: 500px;">
  
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- part was obtained through de novo DNA synthesis
 
  
 
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DNA synthesis
 
DNA synthesis
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===References===
 

Latest revision as of 21:15, 27 August 2015

RBS-GAD-6xHis-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 658
    Illegal AgeI site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- human CHAT gene (UniProt accession no: Q99259) was codon-optimized for E.coli and provided with C-terminal 6xHis tag
- illegal restriction enzyme sites were removed
- part was obtained through de novo DNA synthesis



Source

DNA synthesis