Difference between revisions of "Part:BBa K1598003:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | - human CHAT gene (UniProt accession no: P28329) was codon-optimized for E.coli and provided with C-terminal 6xHis tag | + | <html> |
− | - due to its length, the sequence was synthesized as two gBlock fragments with 30 bp overlap between each other and with 20 bp overlaps with respective PSB1C3 ends | + | - human CHAT gene (UniProt accession no: P28329) was codon-optimized for E.coli and provided with C-terminal 6xHis tag<br> |
+ | - due to its length, the sequence was synthesized as two gBlock fragments with 30 bp overlap between each other and with 20 bp overlaps with respective PSB1C3 ends<br> | ||
- the construct was cloned into PSB1C3 using Gibson Assembly | - the construct was cloned into PSB1C3 using Gibson Assembly | ||
− | + | <br> | |
− | + | </html> | |
===Source=== | ===Source=== |
Latest revision as of 21:05, 27 August 2015
RBS-ChAT-6xHis-Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 682
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 303
Illegal XhoI site found at 1770 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1095
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1765
Design Notes
- human CHAT gene (UniProt accession no: P28329) was codon-optimized for E.coli and provided with C-terminal 6xHis tag
- due to its length, the sequence was synthesized as two gBlock fragments with 30 bp overlap between each other and with 20 bp overlaps with respective PSB1C3 ends
- the construct was cloned into PSB1C3 using Gibson Assembly
Source
DNA synthesis