Difference between revisions of "Part:BBa K1615001:Design"
(→Design Notes) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Morphine dehydrogenase | + | Morphine dehydrogenase is RFC25 compatible. Fusion was performed using RFC10 standard due to illegal NgoMIV site in F1 origin. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 17:48, 27 August 2015
Morphine-6-dehydrogenase driven by LacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2493
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
Illegal NgoMIV site found at 1648 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Morphine dehydrogenase is RFC25 compatible. Fusion was performed using RFC10 standard due to illegal NgoMIV site in F1 origin.
Source
Morphine dehydrogenase from Pseudemonas putida. Lac expression cassette from iGEM10_Washington.