Difference between revisions of "Part:BBa K1755001"
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TIEDLVAYRQ AHERKAS | TIEDLVAYRQ AHERKAS | ||
| − | [[File:LZU_iGEM_2015_ribB_picture.JPG|400px | + | [[File:LZU_iGEM_2015_ribB_picture.JPG|400px|left|Normal E.coli(left one)and E.coli with ribB(right one)]] |
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Revision as of 07:23, 27 August 2015
ribB coding sequence
ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase。 We get this part from E.coli genome by PCR. It can increase the electric output by synthesis of riboflavin.
Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.
Usage and Biology
The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.
Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
Catalytic activity
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.
Sequence
10 20 30 40 50 60 70 80 90 100
TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM
110 120 130 140 150 160 170 180 190 200
GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR
210
TIEDLVAYRQ AHERKAS
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 387
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 319 - 1000COMPATIBLE WITH RFC[1000]
references
UniPort P0A7J0 RIBB_ECOLI [http://www.uniprot.org/uniprot/P0A7J0]